Virus inactivation by aHP was comparable to the decontamination of commercial spore-based biological indicators. External National Institute for Occupational Safety & Health (NIOSH) certification verified respirator structural integrity and filtration efficiency after 10 rounds of aHP treatment. This study provided experimental validation of an aHP treatment process that decontaminates the respirators while maintaining N95 function. For pathogens transmitted via respiratory droplets and aerosols, it is critical to address respirator safety for reuse. In parallel, we assessed the ability of aHP treatment to inactivate multiple viruses absorbed onto respirators, including phi6 bacteriophage, herpes simplex virus 1 (HSV-1), coxsackievirus B3 (CVB3), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Multiple N95 respirator models were subjected to 10 or more cycles of respirator decontamination, with a select number periodically assessed for qualitative and quantitative fit testing. This system is designed to dispense a consistent atomized spray of aerosolized, 7% hydrogen peroxide (H 2O 2) solution over a treatment cycle. In response to the demand for N95 respirators by health care workers during the COVID-19 pandemic, we evaluated decontamination of N95 respirators using an aerosolized hydrogen peroxide (aHP) system. Even on this partial aHP decontamination, this sensitive detection method revealed only three wells (two of which are shown above) with any viral plaques (indicated by arrowheads these equate to 25 PFU of HSV-1 and 5 PFU of CVB3 see plots in Fig. 3 and and4). ![]() The plate shown in panel B is from an aHP cycle run with modification 1 parameters, when no dwell time was used (aHP cycle 3 see Tables 3 and and4), 4), and commercial spore-based biological indicators indicated a failure of decontamination. The plate shown in panel A illustrates serial dilution of a high concentration of HSV-1 after drying only (10 5 PFU see plot in Fig. 3) and a lower concentration of CVB3 after drying only (10 2 PFU see plot in Fig. 4). Viral plaques, which are visible as clear foci of infection (PFU) on the background of methylene blue-stained cells, were visualized at 72 h postinfection. Shown here are representative examples of serial dilutions (titration) of HSV-1 or CVB3 that were spotted onto 3M 9211+ respirators and then resuspended and plated onto monolayers of Vero detector cells. Viral titration demonstrates inactivation and loss of infectious units due to drying and aerosolized H 2O 2 (aHP) decontamination.
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